上海千花mm自荐

On Saturday, Al-Qassam Brigades, the armed wing of the Islamic Resistance Movement (Hamas), launched a large-scale surprise attack on Israel, resulting in a significant number of casualties and penetrating multiple military bases and Israeli settlements. Israel immediately declared that Israel is now "at war" and vowed to "take revenge" on the Hamas militants. This event is undoubtedly the most serious conflict between Israel and Palestine in over a decade, with both sides trapped in a vicious cycle of violence, jeopardizing the fragile geopolitical stability in the Middle East.

The re-eruption of the Israel-Palestine conflict is regrettable, and both sides bear responsibility. Israel's illegal occupation of Palestinian territories and its refusal to accept the "two-state solution" serve as the root causes of the conflict. However, new dynamics in the Palestinian, regional and international situation have acted as the trigger for this latest outbreak.

Firstly, Hamas launched the attack in retaliation for the hardline policies and extremist actions of Israel's far-right government against the Palestinians. Since the Netanyahu government came to power, it has consistently challenged the Palestinian redline and ultimately fueled Palestinian anger. Hamas named this military operation the "Al-Aqsa Flood," highlighting its religious significance and revengeful intent.

Secondly, Hamas sought to counter the marginalization of the Palestinian issue. Recently, Saudi Arabia has been negotiating a "normalization" deal with Israel and has come close to an agreement brokered by the US. Saudi Arabia holds a prominent position in both the Arab and Islamic worlds, and once it establishes diplomatic relations with Israel, the Palestinian cause is likely to be greatly impacted.

Furthermore, Hamas aimed to maintain its leadership position in the armed resistance movement of Palestine. In recent years, Palestinian armed resistance has been led primarily by emerging small-scale militant groups like the "Lions' Den" in the West Bank, challenging Hamas' leadership among Palestinians. It was necessary for Hamas to defend its leadership position through a major, attention-grabbing attack.

Lastly, the economic situation in the Gaza Strip was on the brink of collapse, motivating Hamas to seek a way out for survival. 

Judging from the background of the incident, it is clear that the US also has a looming presence and certain responsibility in the bloody conflict.

First of all, the US abandoned justice by supporting Israel in its conflict with Palestine and condoning Israel's behaviors, which eventually led to this tragedy. Since the beginning of this year, the US, which claims to be the mediator for peace in the Middle East and the most important ally of Israel, has not fulfilled its obligation to promote justice and prevent tensions from escalating. Instead, it has become an accomplice in stirring up the conflict between Palestine and Israel.

Second, the Biden administration has chosen a time like this to desperately push for the normalization of relations between Saudi Arabia and Israel, with the main purpose of boosting Biden's votes in next year's election. It has been acting poorly in Middle East affairs. Therefore, the Biden administration is trying to expand the results of the Abraham Accords to highlight the "fruits of peace" in the Middle East. However, such a "peace in the Middle East" that the US has been showing off has led to an unprecedented threat to the survival of the Palestinians and ultimately led to a bloody conflict. The current escalation of tensions and violence proves that a Middle East peace plan without the two-state solution will only intensify conflicts and hinder peace.

Finally, the US has its own intentions by pushing to improve relations between the Arab countries and Israel - to establish a new political and military alliance against Iran, enhance its ability to control the situation in the Middle East, and, take a longer view, to try to create a coterie to marginalize China's influence in the Middle East.

The "peace" promoted by the US in the Middle East is likely to bring more divergences, contradictions and conflicts to the region. If the US continues to have a bias toward Israel, it will lead the peace process in the Middle East astray, and the Israel-Palestine conflict will recur once and again, while peace in the Middle East will become only castles in the air.

Three of China's science and technology (S&T) clusters are among the world's five biggest S&T clusters as announced by the World Intellectual Property Organization (WIPO).

Wang Wenbin, spokesperson of the Foreign Ministry, said on Monday during a routine press conference that the achievement showed the success of China's measures to encourage relevant regions to leverage their strength as reservoirs of innovation factors and increase their ability to innovate and boost economic growth.

On September 20, the WIPO released the Global Innovation Index 2023, which showed that three Chinese S&T clusters - Shenzhen-Hong Kong-Guangzhou, Beijing as well as Shanghai-Suzhou - were among the world's top five.

In addition, the GII identified 24 S&T clusters in China, up from 21 in 2022, as the country is now home to the greatest number of S&T clusters, said the WIPO.

Wang said that the achievement showed that relevant regions have used their advantages of innovative factors, and this is having a positive effect.

"Since the first edition of the GII was released in 2007, China has been on the list at various ranks more and more frequently, reflecting the continuous improvement of China's innovation-driven development," said Wang.

Data from the National Bureau of Statistics showed that China's innovation-driven index reached 336.3 in 2022, up 15.5 percent year-on-year, and social research and development investment surpassed 3 trillion yuan ($410.37 billion) for the first time, ranking second worldwide, said Wang. 

Daren Tang, director-general of the WIPO, said that China has become a major contributor to international intellectual property work, and has successfully transformed itself into a global center of innovation, creativity and technology.

Wang reaffirmed that innovation is attached with international cooperation, openness and sharing. "China will keep implementing win-win strategy of opening-up, improving openness, promoting international communication and cooperation in science and technology, and building an open, fair, just and non-discriminatory environment for development," he noted.

Shares of the heavily indebted Evergrande Group closed up about 28 percent on Tuesday as the company resumed its trade on the Hong Kong Stock Exchange, several days after its founder Hui Kai Yan has been subject to mandatory measures in accordance with the law due to suspicion of violating law and committing crimes.

Shares of Evergrande jumped as much as 42 percent during Tuesday’s trading. Another Evergrande stock, Evergrande Property Services Group, closed down about 3 percent. 

Real estate and property management services stocks fell on Tuesday’s closing, with Zhenro Properties Group dropping about 9 percent and Country Garden Services Holdings Co falling about 7 percent.

Last week, China Evergrande said it received notification from relevant authorities that Hui Ka Yan, an executive director of the company and chairman of the board of directors of the company, has been subject to mandatory measures in accordance with the law due to suspicion of criminal wrongdoing.

"There is currently no other inside information in relation to the company that needs to be disclosed," Evergrande said in a statement to the Hong Kong Stock Exchange on Monday. 

In September, several staff working in the wealth management unit of the crisis-hit property developer were placed under a criminal probe.

“The resumption of trading of two Evergrande stocks today has attracted much attention, on the one hand, because investors are concerned about Evergrande's current operating conditions and capital market performance, on the other hand, there is no trading in the A-share market due to holidays, and the Hong Kong stock market is more able to see the market performance of real estate stocks,” Yan Yuejin, a research director at Shanghai-based E-house China R&D Institute, told the Global Times on Tuesday.

Yan noted that Evergrande shares had better-than-expected performance, which was in line with the laws of the capital market. Investors will continue to pay close attention to the situation of Evergrande's real estate projects, the expansion of its property services sector and the development direction of new-energy vehicles, he added.

Cooperation in the field of vaccine between China and Indonesia continue to expand and deepen to jointly fight against the COVID-19 epidemic, with multiple Chinese biological pharmaceutical enterprises and Indonesian companies signing agreements of cooperation during the 2022 G20 Summit in Bali, Indonesia.

During the summit, Indonesian biological pharmaceutical company PT Etana Biotechnologies Indonesia (Etana) signed agreements with three Chinese companies including CanSino Biologics Inc, Walvax Biotechnology Co, and Abogen Biosciences.

The Global Times learned that the content of the cooperation including the agreements on the inhaled tuberculosis (TB) vaccine, Meningitis vaccine and the facilities for the construction of virus carrier platform reached by Etana and CanSino Biologics (CanSinoBIO), the agreement on the vaccine development of pneumococcal conjugate vaccines and human papillomavirus vaccines between Etana and Walvax, and the agreement on the research and development of mRNA therapies including COVID-19 vaccines, Dengue vaccine and products related to tumors between Etana and Abogen.

According to Nathan Tirtana, founder and CEO of Etana, the company hope to take advantage of this opportunity to help the Indonesian Ministry of Health achieve its goals and ensure the fair distribution of vaccines, medicines and medical devices among different countries, especially the developing countries.

The Global Times learned from CanSinoBIO that the two companies will promote joint development and commercialization of innovative vaccine products based on their respective advantages and help Indonesia build a regional vaccine production center.

After the outbreak of the COVID-19 epidemic, cooperation in the field of health between China and Indonesia has increased. Particularly, the cooperation in vaccine has always been at the forefront of the world.

China was the first country to cooperate with Indonesia in the research and development of vaccines and specific medicines for COVID-19. China has also supported Indonesia in establishing a regional vaccine production center and is one of the largest suppliers of COVID-19 vaccines to Indonesia. Meanwhile, Indonesia was also one of the first countries to provide material assistance to China after the COVID-19 outbreak.

So far, COVID-19 vaccines of multiple Chinese companies including Sinovac Biotech, China National Biotec Group (CNBG), CanSinoBIO and BioKangtai have been approved for use in Indonesia.

Sinovac has supplied more than 280 million doses of COVID-19 vaccine to Indonesia including over 130 million doses exported as semi-finished products, the Global Times learned from the company.

BioKangtai started production of the adenovirus vector vaccine for COVID-19 in February of 2021, and the vaccine was approved for emergency use in Indonesia on October 31, 2021. The company signed a purchase agreement on the vaccine with an Indonesian partner in November 2021. Over 30 million doses of the company's adenovirus vector vaccine were exported to Indonesia in 2021.

In March this year, CanSinoBIO's adenovirus type-5 vector-based COVID-19 vaccine (Ad5-nCoV) was approved by BPOM Indonesia as booster shots following inactivated vaccine, while CNBG's inactivated vaccine, which had previously been approved as emergency use in Indonesia, was also approved there in March as a heterologous booster.

Apart from the cooperation in the combat against COVID-19 between the two countries, Chinese and Indonesian experts and scientists of TB and respiratory infectious diseases also discussed and shared their latest research results on the elimination of TB with innovative vaccine technologies during a forum in Bali on Wednesday.

The technology used in CanSinoBio's adenovirus vector vaccine can also be used in the TB vaccine, the company said at the forum.

Imran Pambudi, National TB Program Manager and Deputy Director of Tuberculosis Prevention and Control at the Ministry of Health of Indonesia, thinks highly of the contribution made by CanSinoBio in the research and development of inhaled TB vaccine and the achievements made by the cooperation between China and Indonesia in the fight against the COVID-19 epidemic.

Chinese researchers have successfully proven the existence of the very massive first-generation stars, which are the oldest stars in the universe. After years of work, they have discovered the chemical signature of these stars for the first time, a breakthrough that holds significant importance for understanding the origins and evolution of stars.

The research findings were published online in the international scientific journal Nature on Wednesday, providing an answer to the longstanding question in the scientific community regarding the existence of the very massive first-generation stars during the early stages of the universe, approximately 13.8 billion years ago.

The researchers from the National Astronomical Observatory of China analyzed over 5 million stellar spectra obtained from the Large Sky Area Multi-Object Fiber Spectroscopic Telescope (LAMOST) in North China's Hebei Province. Through this analysis, they identified a star located in the halo of the Milky Way, approximately 3,327 light-years away from Earth, with a mass approximately half that of the Sun.

This star exhibited a metal-poor characteristic, indicating its alignment with the characteristics of second-generation stars formed after the demise of the first-generation stars.

The first-generation stars were supermassive stars with masses ranging from 140 to 260 times that of the Sun, making them the oldest stars in the universe. These stars existed over 13 billion years ago but had short lifespans of only 3 million years before undergoing supernova explosions, giving rise to the second-generation stars observed by the researchers.

By observing and studying the second-generation star, the researchers will be able to infer the mass and characteristics of its preceding generation of stars, said Xing Qianfan, a deputy researcher from National Astronomical Observatory of China.

Xing said that the research team will utilize the massive dataset from LAMOST to deduce the distribution of stars with different masses in the earliest first-generation stars. This will enable them to explore the evolutionary history of the entire universe and the evolution of stars.

The virus that causes COVID-19 spreads through the air. But just how much virus people breathe out over the course of infection isn’t well-defined.

To pin the numbers down, olfactory researcher Gregory Lane and colleagues analyzed over 300 breath samples from 43 people with COVID-19, following them for nearly three weeks. Levels varied between and within individuals, but some people shed a lot, releasing over 800 copies of viral RNA per minute at times.

On average, participants breathed out 80 copies per minute for a full eight days after symptoms began, the team reports September 8 in a preprint posted at medRxiv.org. Only after that point did the viral particles drop to nearly undetectable levels.
Lane, of Northwestern University Feinberg School of Medicine in Chicago, and colleagues still need to confirm what percentage of that exhaled viral RNA comes from viruses that can still replicate in another person’s body. And scientists don’t yet know how much virus is required for infection.

But, based on the new data, the team estimates that a high shedder could potentially exhale enough virus to infect someone in a closed space in about 20 seconds, making even elevator rides risky. With an average shedder, infection could take a little under four minutes.

The new study, which will be published in eLife, provides valuable information that both nasal swab studies and one-time aerosol experiments cannot, says infectious diseases researcher Kristen Coleman (SN: 7/16/23). Swabs sample only one part of the respiratory tract. And many aerosol experiments measure the amount of virus people spewed while talking, singing or reading aloud, as well as while while breathing, at just a single point during infection (SN: 08/17/21).

Lane’s team devised a simple and cheap tool — essentially a plastic mouthpiece attached to a closed tube — that participants took home with them and breathed into for 10 minutes at a time. That allowed the team to easily collect samples over an extended period of time and track how emissions changed during infection. But the makeshift tool lacks the precision of advanced machines in laboratories and hospitals, says Coleman, of the University of Maryland School of Public Health in College Park. Even the spike of 876 viral RNA copies per minute seen in the new study may be an underestimate by several orders of magnitude, she says.

In the new study, those who reported more severe symptoms tended to emit more virus. Yet even asymptomatic people or those with mild cases breathed out substantial amounts of viral RNA. Both vaccinated and unvaccinated people exhaled similar levels.

The U.S. Centers for Disease Control and Prevention currently recommends that people who are mildly ill or asymptomatic isolate for at least five days and then mask for another five. Lane would offer different advice: “If my friends or family asked me, I’d say you should isolate through day eight.”

Members of a post-disaster reconstruction working group recently walked into the flooded Xinle village, located in Wuchang city of Northeast China's Heilongjiang Province, to investigate and identify flood-hit houses. According to estimates, review of all the homes in 24 townships will complete in three to four days.

In the aftermath of Typhoon Doksuri, Heilongjiang and multiple other regions in China, including Beijing and North China's Hebei Province, have ramped up efforts to restore order for people living in inundated areas. 

In Guyu village, Wuchang city, more than 2,000 mu (133 hectares) of corn has been soaked in water. The local emergency department has transferred large-scale pumping facilities to carry out 24-hour drainage of farmland water. Wuchang has mobilized six post-disaster technical guidance groups to provide technical guidance for villagers in 24 towns and villages for draining field water and spraying pesticides to prevent and control pests and diseases. 

At Sunday midnight, a train carrying 690 tons of Wuchang rice left the local train station. This was the first train of food supplies transported from Wuchang station after the resumption of the railway, which was cut off by flooding. 

People wearing protective gear for disinfection in Shangzhi, one of the seriously stricken cities in Heilongjiang, have completed three rounds of disinfection across more than 320,000 square meters of flood-affected areas.

In response to the flooding of farmland, Hebei Province organized experts and technicians to head to the front lines of the disaster resistance to guide post-disaster agricultural production. At the same time, the province promptly redirected 1.06 million kilograms of seeds of short-growth crops such as cabbage and spinach to prepare for replanting to minimize farmers' losses.

In Zhuozhou, Hebei, four shopping malls and 29 supermarket chains have resumed operation and schools are also stepping up efforts to clean up sludge and disinfect areas to ensure the timely return of students to campus in September. Hebei officials had said earlier that it plans to complete the reconstruction in two years and will take efforts to ensure affected residents are able to return to their homes prior to the approach of winter. 

In Beijing, the lives of local people have gradually resumed. Most recently, six A-level scenic spots in the Fangshan district have reopened. The Fangshan district has labeled regions into three colors based on the severity of flood impact - red, yellow and green - serving as a more efficient method to solve disaster relief work. Officials from Beijing said they have mapped out a plan to improve the city's disaster relief capabilities and the development of flooded regions over the next three years.

Not only have local departments in flood-stricken regions moved quickly to deal with disaster relief, the Ministry of Agriculture and Rural Affairs urged agricultural departments at all levels to make efforts to alleviate disaster and promote vegetable production.

Meanwhile, to deal with future emergent heavy rainfall and floods and improve the adaptability of urban infrastructure to withstand extreme weather, observers have suggested that the impact infrastructure in some high-risk areas may face from extreme rainstorms brought by climate change be re-evaluated to determine whether the existing level of defense needs to be upgraded, as well as to improve the vigilance and preparedness of local departments and strengthen resilience in the face of a changing climate.

Some water control experts said this round of heavy rainfall reminded some places to make up for the shortcomings of local flood control projects. They pointed out some northern cities in recent years have relaxed their vigilance and constructed flood storage areas in a disorderly manner, such as when building houses, locals may take soil from earth dams, which weakens the flood control capacity of levees.

Sun Shao, a senior researcher at Chinese Academy of Meteorological Sciences, told the Global Times that challenges encountered by cities in northern China due to deficient drainage systems, limited river networks, and inadequate urban green space planning, makes them highly vulnerable to flooding during unexpected heavy rainfall.

Cheng Xiaotao, a deputy chief engineer from the China Institute of Water Resources and Hydropower Research (IWHR), told the Global Times that besides flood control projects, effective communication about the severity of extreme weather among meteorological, hydrological and water conservancy departments is crucial. For instance, emergency command departments can compile data about the specific precipitation and flow rate of rivers and inform the public in detail.

As heavy rainfall events intensify, there is a higher probability of sudden flash floods, mudslides, and other disasters, Sun said. He explained that reviewing historical patterns, during the 1950s to 1970s, the main rain belt was concentrated in northern China, which later shifted to southern China in the 1980s and 1990s. Since the beginning of the 21st century, the main rain belt has gradually moved northward again.

China is considering holding degree holders who use artificial intelligence (AI) to ghostwrite their theses legally responsible. The draft of the Degree Law was submitted to the Standing Committee of the 14th National People's Congress, China's top legislature, for deliberation on Monday.

The draft lays out legal responsibilities for actions such as degree holders using or impersonating another's identity to gain admission qualifications, employing artificial intelligence to author thesis papers, and institutions granting degrees unlawfully, as reported by the media on Monday. 

Academic misconduct includes plagiarism, forgery, data falsification, using artificial intelligence to produce a thesis, impersonating another's identity to obtain admission qualifications, and securing admission qualifications and graduation certificates through illicit means like favoritism and cheating. The draft also addresses other illegal or irregular behaviors that, when exhibited during the study period, should prevent the awarding of a degree.

The draft states that if an individual who has already obtained a degree is found to have used illegal means to do so, the degree-granting institution must revoke the degree certificate. This decision should be made following a review by the degree evaluation committee.

House dust mites surround us. Burrowing cheerfully into our pillowcases, rugs and furniture, the mites feast on our dead skin cells, breaking them down into small particles they can digest.

Now that your skin is crawling, relax. If you’re like most people, you will never know they are there.

An unlucky minority, however, is very aware of dust mites. Some of these unfortunate folks have a simple dust allergy. But others have an additional condition called atopic dermatitis, often referred to as eczema. They react to the presence of dust — or rather, dust mites — with hideous itching and redness. It wasn’t totally clear what, exactly, caused people with dermatitis to react so badly to dust mites.

It turns out that these people react not to the dust mite, but to its dinner — to the breakdown products of the person’s own skin. The finding helps explain why people with atopic dermatitis react so badly to dust mites, and it provides several new options to help treat the itch. It also resolves a decade-long debate in dermatology — why people with dermatitis are scratching in the first place.

Inside out vs. outside in
Atopic dermatitis is known for producing red, cracked and dry skin and, of course, the itching. People usually get diagnosed in childhood. Sometimes it goes away as kids get older, but it still affects between 9 and 30 percent of adults in the United States. Patients with dermatitis who react to dust are told to avoid dusty places and use special pillowcases. For the worst outbreaks, they are often prescribed a steroid cream. In some cases, they can end up in the hospital.

But what causes the itch in the first place? For the past 10 years, scientists have been scratching away at two hypotheses — one called “inside out,” and the other called “outside in.”

The “inside out” hypothesis came first, explains Graham Ogg, a dermatologist with the Medical Research Council Human Immunology Unit at the University of Oxford. The idea was that the immune system was overreacting to normal things: Dermatitis was an inside problem with the immune system itself.

In 2006, however, researchers reported in Nature Genetics that deficiencies in a protein called filaggrin were associated with atopic dermatitis. Now, it’s estimated that 20 to 30 percent of people with atopic dermatitis are also deficient in filaggrin, a protein in the outermost layer of the skin.

“It’s important for moisturizing the skin, keeping the skin hydrated,” explains Ogg. If people with dermatitis are deficient in filaggrin, then “the primary problem isn’t the immune system, it’s the barrier function in the skin.” If the barrier breaks down, more irritants can get in, prompting the immune response and the intolerable itch. So, the “outside in” hypothesis was born. In this view, the immune system wasn’t overreacting; instead it was reacting properly to the avalanche of aggravations it was faced with.

But what if these two hypotheses weren’t at odds, Ogg wondered, and instead were two sides of the same coin? To find out, Ogg and his group began by looking at a molecule called CD1a. This molecule is produced in the skin, and specializes in presenting bits of foreign matter to T cells — the immune system responders that mount attacks against foreign invaders.

It turns out that the CD1a molecules responded to extract-of-house-dust-mite — the delightful concoction that people get scratched with when they are tested for a dust allergy. And when they react, it’s because of CD1a molecules.

To find out if people with dermatitis had more CD1a than people without the condition, the scientists used suction to give eczema sufferers and healthy volunteers large blisters on their arms. The blisters were harvested for their skin and blood cells. And in patients with atopic dermatitis, those skin and blood cells were stuffed with CD1a, far more than in healthy controls.

But what was the CD1a reacting to? Usually CD1a senses fat molecules, presenting bits of them to the immune system to prep it for attack. Ogg and his group assumed that if they analyzed house dust mites, they would find the lipid or fat responsible.
Not quite. Instead, they found a protein called phospholipase A2. Phospholipase is an enzyme that dust mites produce that breaks down skin cells, producing fat molecules the mites can digest. CD1a, it turns out, responds to those lipids — reacting to the house dust mite’s dinner. Reacting, really, to the breakdown products of human skin.

This seems like support for the “inside out” hypothesis. CD1a is part of the immune system, and the immune system does seem to be over-reacting.

Filaggrin also had a role to play. The protein doesn’t just create a barrier to keep the skin moisturized — it’s also anti-inflammatory, Ogg’s group showed. If a skin sample was challenged with essence of dust mite, adding filaggrin could damp down the immune response. But eczema patients with low or no filaggrin had no defense. Their skin was more permeable, and there was nothing to stop the inflammation. The “outside in” hypothesis —the idea that the barrier function is the broken part of the system – is true too. Ogg and his colleagues report their findings February 10 in Science Translational Medicine.

“It links together the observations very nicely,” says Muzalifah Haniffa, a dermatologist at Newcastle University in England. It never was a matter of “inside out” or “outside in.” The two are inextricably linked.

Eat like a dust mite, sting like a bee?
So, to recap: As dust mites chow down on human skin, they cause damage to the cells. People with dermatitis have immune systems that detect the products of the damage and react, causing itching and pain. Filaggrin, when present, can tamp down the response. But when absent, nothing stops the itch.

The study shows filaggrin is far more than a simple barrier protein. Instead it directly affects immune responses in the skin, something that’s never been seen before, Haniffa notes.

This isn’t the first time that Ogg’s group has come across phospholipase A2. “Bee venom also contains phospholipase. In fact it contains massive amounts,” Ogg explains. Knowing that bee venom and dust mites have something in common helps scientists to understand one of the ways that the immune system senses damage to skin — and gives them another option to consider for treatment.

Right now, clinical trials are focused on stopping the inflammatory proteins produced further down the line. But, Haniffa says, scientists might try methods to increase the amount of filaggrin in the skin — beefing up the barrier against dust mite incursions and reducing the immune response at the same time. Other drugs or creams could target phospholipase A2, inactivating it. Without phospholipase, dust mites wouldn’t be able to break down skin cells, halting any immune reaction.

And that means we can hope for a new day. One with, hopefully, no itch.

New cancer-fighting nanoparticles deliver results — and status reports.

Tiny biochemical bundles carry chemotherapy drugs into tumors and light up when surrounding cancer cells start dying. Future iterations of these lab-made particles could allow doctors to monitor the effects of cancer treatment in real time, researchers report the week of March 28 in the Proceedings of the National Academy of Sciences.

“This is the first system that allows you to read out whether your drug is working or not,” says study coauthor Shiladitya Sengupta, a bioengineer at Brigham and Women’s Hospital in Boston.
Each roughly 100-nanometer-wide particle consists of a drug and a fluorescent dye linked to a coiled molecular chain. Before the particles enter cells, the dye is tethered to a “quencher” molecule that prevents it from lighting up. When injected into the bloodstream of a mouse with cancer, the nanoparticles accumulate in tumor cells and release the drug, which activates a protein that tears a cancer cell apart. This cell-splitting protein not only kills the tumor cell, but also severs the link between the dye and the quencher, allowing the nanoparticles to glow under infrared light.

Previous techniques could track drugs entering tumors, but that “doesn’t necessarily tell you whether the drug is working or not,” says study coauthor Ashish Kulkarni, a bioengineer at Brigham and Women’s and at Harvard Medical School.
The team tested the nanoparticles in mice that each had two types of tumor: one resistant to the drug in the particles and one responsive to the drug. Drug-sensitive tumors glowed around five times as intensely as the resistant tumors. Results were swift, with tumors lighting up in eight to 12 hours.
Replacing the particles’ cancer drug with antibodies that summoned the body’s tumor-fighting defenses allowed the team to test the nanoparticles as immunotherapy agents. In this case, tumors lit up after five days, reflecting an initial lag time of immunotherapy compared with chemotherapy.

These nanoparticles are a proof of concept, Sengupta says. Next steps include redesigning the nanoparticles using clinically approved materials and dyes that would be easier to track in the human body with the use of an MRI machine. But such imaging chemicals can be toxic, which could pose a problem for the nanoparticle design, says cancer nanotechnologist Mansoor Amiji of Northeastern University in Boston. Dyes should be cleared from the body as quickly as possible, while the drug they’re paired with might take weeks to work. But the study’s focus on detecting drug performance in real time is very important, and demands further study, Amiji says. “There’s tremendous need, especially as we think about personalizing cancer therapies.”