An elephant may be hundreds of times larger than a cat, but when it comes to pooping, it doesn’t take the elephant hundreds of times longer to heed nature’s call. In fact, both animals will probably get the job done in less than 30 seconds, a new study finds.
Humans would probably fit in that time frame too, says Patricia Yang, a mechanical engineering graduate student at the Georgia Institute of Technology in Atlanta. That’s because elephants, cats and people all excrete cylindrical poop. The size of all those animals varies, but so does the thickness of the mucus lining in each animal’s large intestine, so no matter the mammal, everything takes about the same time — an average of 12 seconds — to come out, Yang and her colleagues conclude April 25 in Soft Matter.
But the average poop time is not the real takeaway here (though it will make a fabulous answer to a question on Jeopardy one day). Previous studies on defecation have largely come from the world of medical research. “We roughly know how it happened, but not the physics of it,” says Yang.
Looking more closely at those physical properties could prove useful in a number of ways. For example, rats are often good models for humans in disease research, but they aren’t when it comes to pooping because rats are pellet poopers. (They’re not good models for human urination, either, because their pee comes out differently than ours, in high-speed droplets instead of a stream.)
Also, since the thickness of the mucus lining is dependent on animal size, it would be better to find a more human-sized stand-in. Such work could help researchers find new treatments for constipation and diarrhea, in which the mucus lining plays a key role, the researchers note.
Animal defecation may seem like an odd topic for a mechanical engineer to take on, but Yang notes that the principles of fluid dynamics apply inside the body and out. Her previous research includes a study on animal urination, finding that, as with pooping, the time it takes for mammals to pee also falls within a small window. (The research won her group an Ig Nobel Prize in 2015.)
And while many would find this kind of research disgusting, Yang does not. “Working with poop is not that bad, to be honest,” she says. “It’s not that smelly.” Plus, she gets to go to the zoo and aquarium for her research rather than be stuck in the lab. But the research does involve a lot of poop — and watching it fall. For the study, the researchers timed the how long it took for animals to defecate and calculated the velocity of the feces of 11 species. They filmed dogs at a park and elephants, giant pandas and warthogs at Zoo Atlanta. They also dug up 19 YouTube videos of mammals defecating. Surprisingly, there are a lot of those videos available, though not many were actually good for the research. “We wanted a complete event, from beginning to end,” Yang notes. Apparently not everyone interested in pooping animals bothers to capture a feces’ full fall.
The researchers also examined feces from dozens of mammal species. (They fall into two classes: Carnivores defecate “sinkers,” since their feces are full of heavy indigestible ingredients like fur and bones. Herbivores defecate less-dense “floaters.”) And they considered the thickness and viscosity of the mucus that lines mammals’ intestines and helps everything move along as well the rectal pressure that pushes the material. All this information went into a mathematical model of mammal defecation — which revealed the importance of the mucus lining.
Yang isn’t done with this line of research. The model she and her colleagues created applies only to mammals that poop like we do. There’s still the pellet poopers, like rats and rabbits, and wombats, whose feces look like rounded cubes. “I would like to complete the whole set,” she says. And, “if you’ve got a good team, it’s fun.”
A question flamingo researchers get asked all the time — why the birds stand on one leg — may need rethinking. The bigger puzzle may be why flamingos bother standing on two.
Balance aids built into the birds’ basic anatomy allow for a one-legged stance that demands little muscular effort, tests find. This stance is so exquisitely stable that a bird sways less to keep itself upright when it appears to be dozing than when it’s alert with eyes open, two Atlanta neuromechanists report May 24 in Biology Letters. “Most of us aren’t aware that we’re moving around all the time,” says Lena Ting of Emory University, who measures what’s called postural sway in standing people as well as in animals. Just keeping the human body vertical demands constant sensing and muscular correction for wavering. Even standing robots “are expending quite a bit of energy,” she says. That could have been the case for flamingos, she points out, since effort isn’t always visible. Ting and Young-Hui Chang of the Georgia Institute of Technology tested balance in fluffy young Chilean flamingos coaxed onto a platform attached to an instrument that measures how much they sway. Keepers at Zoo Atlanta hand-rearing the test subjects let researchers visit after feeding time in hopes of catching youngsters inclined toward a nap — on one leg on a machine. “Patience,” Ting says, was the key to any success in this experiment.
As a flamingo standing on one foot shifted to preen a feather or joust with a neighbor, the instrument tracked wobbles in the foot’s center of pressure, the spot where the bird’s weight focused. When a bird tucked its head onto its pillowy back and shut its eyes, the center of pressure made smaller adjustments (within a radius of 3.2 millimeters on average, compared with 5.1 millimeters when active). Museum bones revealed features of the skeleton that might enhance stability, but bones alone didn’t tell the researchers enough. Deceased Caribbean flamingos a zoo donated to science gave a better view. “The ‘ah-ha!’ moment was when I said, ‘Wait, let’s look at it in a vertical position,’” Ting remembers. All of a sudden, the bird specimen settled naturally into one-legged lollipop alignment.
In flamingo anatomy, the hip and the knee lie well up inside the body. What bends in the middle of the long flamingo leg is not a knee but an ankle (which explains why to human eyes a walking flamingo’s leg joint bends the wrong way). The bones themselves don’t seem to have a strict on-off locking mechanism, though Ting has observed bony crests, double sockets and other features that could facilitate stable standing.
The bird’s distribution of weight, however, looked important for one-footed balance. The flamingo’s center of gravity was close to the inner knee where bones started to form the long column to the ground, giving the precarious-looking position remarkable stability. The specimen’s body wasn’t as stable on two legs, the researchers found. Reinhold Necker of Ruhr University in Bochum, Germany, is cautious about calling one-legged stances an energy saver. “The authors do not consider the retracted leg,” says Necker, who has studied flamingos. Keeping that leg retracted could take some energy, even if easy balancing saves some, he proposes.
The new study takes an important step toward understanding how flamingos stand on one leg, but doesn’t explain why, comments Matthew Anderson, a comparative psychologist at St. Joseph’s University in Philadelphia. He’s found that more flamingos rest one-legged when temperatures drop, so he proposes that keeping warm might have something to do with it. The persistent flamingo question still stands.
Astronomers want you in on the search for the solar system’s ninth planet.
In the online citizen science project Backyard Worlds: Planet 9, space lovers can flip through space images and search for this potential planet as well as other far-off worlds awaiting discovery.
The images, taken by NASA’s Wide-field Infrared Survey Explorer satellite, offer a peek at a vast region of uncharted territory at the far fringes of the solar system and beyond. One area of interest is a ring of icy rocks past Neptune, known as the Kuiper belt. Possible alignments among the orbits of six objects out there hint that a ninth planet exerting its gravitational influence lurks in the darkness (SN: 7/23/16, p. 9). The WISE satellite may have imaged this distant world, and astronomers just haven’t identified it yet. Dwarf planets, free-floating worlds with no solar system to call home (SN: 4/4/15, p. 22) and failed stars may also be hidden in the images. The WISE satellite has snapped the entire sky several times, resulting in millions of images. With so many snapshots to sift through, researchers need extra eyes. At the Backyard Worlds website, success in spotting a new world requires sharp sight. You have to stare at what seems like thousands of fuzzy dots in a series of four false-color infrared images taken months to years apart and identify faint blobs that appear to move. Spot that movement and you may have found a new world.
But you can’t let blurry spots or objects moving in only a couple of the frames fool you: Image artifacts can look like convincing space objects. True detections come from slight shifts in the positions of red or whitish-blue dots. With so many dots to track, it’s best to break up an image into sections and then click through the four images section by section. This process can take hours. But think of the payoff — discovering a distant world no one has observed before.
Once you’ve marked any potential object of interest, the project’s astronomers take over. Jackie Faherty of the American Museum of Natural History in New York City and colleagues cross-reference the object’s coordinates with databases of celestial worlds. If the object does, in fact, appear to be a newbie, the team requests time on other telescopes to do follow-up. Those studies can reveal whether the object is a failed star or a planet.
So far, tens of thousands of citizen scientists have scoured images at Backyard Worlds. The team has identified five possible failed stars and had its first paper accepted for publication.
But there’s still much more to explore: The elusive Planet Nine might still be out there, disguised as a flash of dots.
After an influenza infection, the nose recruits immune cells with long memories to keep watch for the virus, research with mice suggests.
For the first time, this type of immune cell — known as tissue resident memory T cells — has been found in the nose, researchers report June 2 in Science Immunology. Such nasal resident memory T cells may prevent flu from recurring. Future nasal spray vaccines that boost the number of these T cells in the nose might be an improvement over current flu shots, researchers say. It’s known that some T cell sentinels take up residence in specific tissues, including the brain, liver, intestines, skin and lungs. In most of these tissues, the resident memory T cells start patrolling after a localized infection. “They’re basically sitting there waiting in case you get infected with that pathogen again,” says Linda Wakim, an immunologist at the University of Melbourne in Australia. If a previous virus invades again, the T cells can quickly kill infected cells and make chemical signals, called cytokines, to call in other immune cells for reinforcement. These T cells can persist for years in most tissues.
It’s different in the lungs. There, resident memory T cells have shorter-term memories than ones that reside in other tissues, scientists have previously found. To see if all tissues in the respiratory tract have similarly forgetful immune cells, Wakim and colleagues tagged immune cells in mice and sprayed flu virus in the rodents’ noses. After infection, resident memory T cells settled into the nasal tissue. The researchers haven’t yet dissected any human noses, but it’s a pretty good bet they also contain resident memory T cells, Wakim says. Unlike in the lungs, the nose T cells had long memories, persisting for a least a year. “For mice, that’s quite a long time, almost a third of their life,” Wakim says. She doesn’t yet know why there’s a difference between nose and lung T cell memories, but finding out may enable researchers to boost lung T cell memory. Still, with nose T cells providing security, the lungs might not need much flu-fighting memory. Memory T cells that patrol only the upper respiratory tract could stop viruses from ever reaching the lungs, Wakim’s team found. An injection of virus under the skin didn’t produce any resident memory T cells in the respiratory tract. Those findings could mean that vaccines delivered via nasal spray instead of shots might stimulate memory T cell growth in the nose and could protect lungs from damage as well. A nasal spray called FluMist has had variable results in people. No one knows if that vaccine can produce nasal memory T cells. It’s not surprising to find that the nose has its own resident memory T cell security force, says Troy Randall, a pulmonary immunologist at the University of Alabama at Birmingham. “But it’s a good thing to know and certainly they’re the first to show it.”
The discovery may direct some research away from the lungs and toward the nose, Randall says. Future research should focus on how the resident memory T cells work with memory B cells that produce antibodies against viruses and bacteria, he suggests.
Water swirling down a drain has exposed an elusive phenomenon long believed to appear in black holes.
Light waves scattering off a rotating black hole can bounce off with more energy than they came in with, by sapping some of the black hole’s rotational energy. But the effect, predicted in 1971 and known as rotational superradiance, is so weak that it would be extremely difficult to observe in a real black hole. So scientists had never seen rotational superradiance in action. Now, physicists report June 12 in Nature Physics that they’ve glimpsed the effect for the first time, in a black hole doppelgänger made with a vortex of water, similar to water swirling down a bathtub drain. “If you take a tennis ball and you throw it against a wall, you don’t expect it to come back with more energy,” says Silke Weinfurtner of the University of Nottingham in England, who led the study. “But when you throw something at a black hole, if it’s a rotating black hole, you can actually gain energy.”
To demonstrate the effect, the scientists created a swirl of water. “The fluid has to drain in a way that looks like a black hole,” says physicist Antonin Coutant, also at Nottingham. Surface ripples reach a point of no return where they are sucked into the vortex. That’s analogous to a black hole’s event horizon, the boundary from which no light can escape. Weinfurtner, Coutant and colleagues report that water waves scattering off the vortex got a superradiant boost: They were amplified by up to 14 percent on average, depending on the frequency and direction of the waves.
For obvious reasons, researchers can’t study a real black hole in a laboratory. If they could, “we’d all be in trouble,” says physicist Sam Dolan of the University of Sheffield in England, who was not involved with the study. A water vortex is the next best thing. The result, Dolan says, “gives us more confidence that our theories about black holes are correct.”
Although rotational superradiance is a weak effect in black holes, there may be opportunities to observe it, says physicist Vítor Cardoso of Instituto Superior Técnico in Lisbon, Portugal. Superradiance affects gravitational waves as well as light waves. Ripples in spacetime stirred up by merging black holes (SN Online: 6/1/17) should be slightly amplified if those black holes are spinning. That amplification could be observed by future ultrasensitive gravitational wave detectors.
For the first time, researchers have watched relatively cool parcels of plasma speed away from the surface of the sun and off into space, all the while cocooned in a million-degree flare.
Shadia Habbal of the University of Hawaii in Honolulu and colleagues used a specially designed spectrometer to observe the eruption from Svalbard, Norway, during the March 2015 solar eclipse. The results, published online June 9 in Astrophysical Journal Letters, include measurements of the speed, temperature and composition of filaments of solar material streaming away from the sun — three features never measured simultaneously before. The data provide the first direct evidence of cooler solar material within an eruption and map its speed and trajectory, says Enrico Landi of the University of Michigan in Ann Arbor. “No instrument has ever been able to obtain these data.”
The sun’s surface is a roiling mass of hot ionized gas, or plasma, which is about a roasty 6,000° Celsius. But its corona, the wispy, halolike atmosphere that is visible during a solar eclipse, is superheated to millions of degrees. Scientists are still not sure how it gets so hot.Every so often, a huge, hot bubble of coronal plasma appears to burst off the sun’s surface in an eruption called a coronal mass ejection, or CME. These ejections send energetic charged particles hurtling into space at millions of miles per hour. When aimed at Earth, those particles can damage satellites and knock out power systems (SN Online: 4/9/12), so scientists want to understand CMEs to better predict them. The trouble is, it’s difficult to watch CMEs close to their origins. Sun-watching spacecraft block out the bulk of the sun’s light with a shield to avoid blinding their cameras. That gives spacecraft a constant view of the shimmering corona, but hides the sun’s surface.
The March 20, 2015, total solar eclipse over Svalbard gave Habbal’s team a rare view of the whole solar atmosphere because the moon and the sun appear almost the same size in the sky. “You can see things right from the solar surface out to several solar radii,” she says.
Her team brought a custom-built spectrometer the size of an airline-approved carry-on, designed by coauthor Adalbert Ding of the Technical University of Berlin. The spectrometer is sensitive to wavelengths of light emitted by iron atoms that have lost all but 11 or 14 of their electrons. Researchers can use those iron ions in the solar material to trace temperature: The hotter the solar plasma, the more electrons the iron has lost. As expected, coronal temperatures soared to 2 million or so degrees Celsius. But the team also saw some cooler blobs at a mere 20,000° C. Instead of losing their electrons to the heat and becoming ionized, these blobs maintained their cool.
Habbal thinks these are bright fingers of plasma called prominences, which had previously been observed stretching away from the sun before a CME.
“If you have an ice cube in a hot bath, it’s going to melt and evaporate,” Habbal says. “Here you have clusters of cool material that are enwrapped by very hot material. You would expect them to get ionized, but they didn’t.”
By measuring the solar material’s Doppler shift, or the change in wavelength as the material moved, the instrument could also clock its speed and direction. If the wavelength of the light appeared longer (or redder) in some places, that means the material was moving away from the observers. Shorter wavelength, or bluer light, indicated material moving toward them.
During the eclipse, material around the solar disk zoomed away at 100 to 1,500 kilometers per second. Such great speeds indicated that a CME erupted, and the direction suggested it was on the far side of the sun. Catching a CME during the short eclipse was a lucky coincidence.
“Lots of things about this experiment were just sheer luck,” Habbal says.
Cool inclusions have been spotted inside the hot corona before, but this is the first time they were seen fleeing the surface of the sun during a CME, Habbal says.
Prominences are thought to be associated with CMEs, and might even trigger their eruptions, but no one is sure how. Now that it’s possible to trace them from the solar surface out into space, researchers hope to spot more during the total eclipse as it crosses the United States in August (SN: 8/20/16, p. 14).
“If successful, it will be the dataset of a lifetime,” says Landi.
Hunter-gatherers who built and worshiped at one of the oldest known ritual centers in the world carved up human skulls in a style all their own.
At Turkey’s Göbekli Tepe site — where human activity dates to between around 11,600 and 10,000 years ago — people cut deep grooves in three human skulls and drilled a hole in at least one of them, say archaeologist Julia Gresky of the German Archaeological Institute in Berlin and colleagues. Ancient hunter-gatherers there practiced a previously unknown version of a “skull cult,” in which human skulls were ritually modified after death and then deposited together, Gresky’s team reports online June 28 in Science Advances.
Collections of human skulls modified in other ways have been found at several sites from around the same time. For instance, deliberately broken faces on skulls were unearthed at a Syrian settlement and may represent a form of punishment after death.
Seven excavated skull fragments enabled Gresky’s group to reconstruct the Göbekli Tepe skulls. These skulls of the recently deceased were carved for use in ceremonies to worship them as ancestors, the researchers propose. It’s also possible that the skull incisions marked deceased individuals who had been especially revered or reviled while alive.
A cord inserted through the hole drilled in one skull may have suspended that skull for display. Grooves probably ran from front to back on the skulls and possibly stabilized cords that held decorations of some kind.
Microscopic study of skull pieces from Göbekli Tepe indicates that grooves were cut with stone tools. A lack of healed bone on the edges of incisions suggests skull carving occurred shortly after death.
In July of 1972, NASA launched the first Landsat satellite into orbit around Earth. Since then, the spacecraft and its successors have transformed our understanding of Antarctica (and the rest of the planet, too). In the first year following the launch, Landsat’s images of the faraway continent showed “uncharted mountain ranges, vast ice movements and errors in maps as little as two years old,” according to an article published in Science News. William MacDonald of the U.S. Geological Survey, who had spent eight years mapping a part of West Antarctica, was “shocked” to learn of previously unknown peaks just 100 miles from McMurdo Station.
Landsat’s images weren’t the first overhead shots of Antarctica, but to this day the program provides researchers a reliable and repeating view of hard-to-reach corners of the planet. It was Landsat images that in November of 2014 first alerted scientists to a growing crack in the Larsen C ice shelf that, after lengthening by about 20 kilometers in less than nine months, threatened to break off a Delaware-sized chunk of the shelf. With thermal imagery from Landsat 8 along with data from the European Space Agency’s Sentinel-1 satellites, scientists sitting half a world away tracked the Larsen C crack to its final break, as described by Ashley Yeager. While satellites are scientists’ eyes in the skies, seismic sensors serve as ears to the ground. Alexandra Witze describes the work of scientists who are using seismic sensors to monitor nuclear weapons activity in a part of the planet where access to information is limited: North Korea. Five nuclear weapons tests have been confirmed in the country since 2006, all at an underground test site in Mount Mantap. By tracking seismic waves produced by such explosions, and comparing these rumbles with each other and with those produced by natural earthquakes and in experimental tests, researchers around the world gain valuable clues to where the hidden explosions are happening and, importantly, how powerful they are. A North Korea weapons test last year was detected as far away as Bolivia.
The art of eavesdropping certainly has its rewards. There are plenty more examples. Rachel Ehrenberg writes about how snooping scientists might listen in on kelp to predict ecosystem health. And Emily Conover reports on a newly discovered, relatively itty-bitty star some 600 light-years away. Astronomers spied on the star by watching it pass in front of a larger star, dimming the larger star’s light.
Sometimes astronomers get lucky and distant phenomena are much more straightforward to study. That will be the case later this month when a total solar eclipse passes across North America from Oregon to South Carolina. People will be monitoring the August 21 eclipse in all sorts of ways, including via a livestream from balloons at the edge of the atmosphere, as Lisa Grossman describes in “Watch the moon’s shadow race across the Earth from balloons.” Grossman will be reporting on the eclipse on the ground with scientists in Wyoming. You’ll find her stories — along with many others about the ways scientists watch, listen and learn — at www.lssfzb.com
Thanks, Holly Gaff. Soon, anyone straining to tweeze off a mid-back tick can find answers to the obvious question: What if humankind just went after the little bloodsuckers with killer robots?
Gaff, who calls herself a mathematical ecoepidemiologist, at Old Dominion University in Norfolk, Va., is one of the few people collecting real field data on the efficacy of tick-slaying robots. This summer, she’s been supervising a field test of a terminator named TickBot deployed to try making mowed grass safe for children. Researchers will start analyzing results in early fall.
Ticks make formidable enemies. “Almost every control measure that has been tried has failed, and has failed miserably,” Gaff says. “We are slowly coming to embrace the fact that you cannot eradicate ticks.” What human ingenuity might do, however, is manage the risks and — dream big! — make ticks irrelevant. That’s an urgent hope. Data from the Cary Institute of Ecosystem Studies in Millbrook, N.Y., have for two years suggested 2017 will be a high risk one for Lyme disease in the Northeastern United States. Of the various illnesses that North America’s ticks pass along, Lyme is the most common, caused by a squiggle of a parasite called Borrelia burgdorferi. The disease can bring on an eerie red bull’s-eye rash, flulike misery and risks of long-term neurological and joint troubles if not treated early. In 2015, the U.S. Centers for Disease Control and Prevention tallied about 30,000 confirmed cases. Considering gaps in case reporting, some estimates put the number closer to several hundred thousand.
So bring on the robots and other science revenge fantasies. It’s time to rethink humankind’s defenses against ticks. Pesticides and tick checks just aren’t doing the trick. There may be ways to attack ticks without touching a single molecule of their die-hard little bodies. Ecologists have made progress in tracing what ticks need from the woods and lawns where they lurk. For instance, researchers believe that it was a bumper crop of acorns in 2015 that, through a Rube Goldberg series of consequences, created conditions for a perfect tick storm two years later. Breaking key ecological connections could knock back the tick menace in the future.
Molecular biologists are focusing on tick survival tricks. Researchers are looking for weak spots inside tick guts and trying to take advantage of ticks’ reckless abandon in mating. Biology is proving as important as electronics in the robot line of defense.
Though, Gaff warns, the top design is not the laser-blazing Armageddon that a recently tick-bitten human might crave. Ticks attack First, a quick intro to ticks.
Unlike mosquitoes, ticks are pure vampires, consuming nothing but blood. Mosquitoes get colloquially called vampires, but blood is just their version of a pregnancy craving, a female-only nutrient gorge to aid reproduction in an adult life of sipping flower nectar. For most of the troublesome tick species in North America, including the black-legged ticks that spread Lyme, blood is the elixir that lets them transition to the next life stage — from larva to nymph to adult. And after a single meal, an adult female can lay 1,000 or even 15,000 eggs without anything else to eat for the rest of her life. Hard ticks, the Ixodidae family, which includes the black-legged variety, typically have only two or three meals of any kind during the entire two or three years they live.
Soft ticks are gluttons, relatively speaking. Many move into mammal dens for a bedbug lifestyle. These ticks hide and, whenever they get hungry, just crawl over to the resident dinner.
For ticks without live-in prey, many “quest,” as the ambush is called. Ticks climb to some promising spot like the top of a grass blade, raise their front legs and just wait until something brushes by. But there are also ticks that hunt vigorously, even pursuing human prey.
A visit to Dennis Bente at the University of Texas Medical Branch in Galveston is unforgettable, in part because of a video of a Hyalomma tick chasing down one of Bente’s collaborators. The tiny brown creature scurries like a frantic ant in an almost-straight line over bare dirt, onto a boot and finally into a hand reaching down to grab it. This hunter doesn’t live in North America.
Ticks can spread a wide variety of diseases. Despite its name, Rocky Mountain spotted fever, which brings a higher risk of fatality than Lyme, is more common in the central United States and the South than in the Rockies. Other tickborne diseases are lately getting attention: A tick-bitten baby in Connecticut in April became the state’s first reported victim of the rare, but potentially fatal Powassan virus, thought to enter the bloodstream in just 15 minutes after a tick starts feeding. And medical journals are publishing discussions of whether a tick bite might lead to a sudden, deadly allergy to red meat. With a possible threat even to our beloved hamburger, new approaches to fending off ticks can’t come soon enough. Super reactors The most dramatic way of rendering a disease irrelevant is a vaccine. One company raised hopes for this approach in April in Washington, D.C., at the World Vaccine Congress by announcing the start of human safety tests of a new Lyme disease formulation. The only Lyme vaccine for humans in the United States was withdrawn voluntarily in 2002 when controversy stalled sales. (Dogs can still get a Lyme vaccination.)
The strategy for the new Lyme vaccine isn’t like the familiar flu or tetanus vaccines because the pathogens get killed outside the human body. The company, Valneva, based in Lyon, France, has redesigned a protein, OspA, used in previous Lyme vaccines. The vaccine trains the human immune system to fight OspA, found on the surface of B. burgdorferi. When a black-legged tick starts sucking human blood, human immune cells get slurped in too and kill the Lyme-causing pathogens before they leave the tick’s gut. “The idea of this vaccine … is vaccinating the tick,” says CEO Thomas Lingelbach. Even if the new vaccine proves to be safe and effective, its first shot in a doctor’s office, in the most optimistic view, is five to 10 years away.
There may be a bigger-picture way to imagine vaccines, however, than targeting each disease with its own shot. Ecologist Richard Ostfeld of the Cary Institute is one of the people hoping for a vaccine that stops the tick itself, and thus all the diseases it may pass along. By the luck of the great lottery of genetics, Ostfeld has a hyperactive immune response to tick saliva. Think of it as a natural version of what a tick vaccine might achieve.
Despite “many, many dozens of tick bites” over his career monitoring Lyme disease risk, Ostfeld has not gotten sick. He often wakes in the middle of the night with a “burning sensation” somewhere on his body. “I … put on my glasses and, sure enough, there’s a little dark spot surrounded by what’s already turned kind of red.” Warned by his vigilant immune system, he pulls off the dark bit of tick, which is usually dead or dying.
Maybe it’s a thing among tick scientists. Sam Telford of Tufts University’s veterinary school in North Grafton, Mass., who also studies the ecology of Lyme disease, has a similar reaction. Bites, he says, “itch like crazy.” A vaccine that makes people itch doesn’t sound very marketable, but blood that somehow poisons ticks sounds good.
A vaccine to protect cattle against debilitating blood loss from bites already targets the tick itself. Newer ways of targeting ticks are being developed for livestock, and for humans, though protecting our species poses extra challenges. Ticks east and west Of the nine or so tick species that spread diseases in North America, the three highlighted below cause the most trouble. Maps show each tick’s U.S. habitat. Fix the landscape “It would be lovely if we could get a vaccine,” says biochemist Kevin Esvelt of MIT. “But there’s a certain elegance to tackling the heart of the problem, which is ecological.” In several papers posted online at bioRxiv.org in 2016 and this year, Esvelt and colleagues laid out an approach that could slash Lyme risks by causing genetic changes in one of the mammals that ticks feed on, changes that could spread across whole landscapes.
The view of Lyme as an ecological disease blames much of the rise in cases on the suburbanization and fragmentation of once-wild countryside in North America. The shifts have fueled population booms in mammals such as white-footed mice that easily become great scurrying reservoirs of Lyme parasites. Ticks gorge on the mouse blood and a high percentage ingest the parasites.
Deer pick up a lot of the Lyme-spreading black-legged ticks. Yet deer aren’t biologically friendly to B. burgdorferi. The mice make a much better parasite paradise than deer do and are more likely to transmit those parasites.
The common name, “deer tick,” was a fluke of early misidentification, Ostfeld says. The tick was easy to collect on deer. But taxonomists realized the hard tick is just a northern form of the long-known black-legged tick, which dines on many mammals. In fact, just how deer abundance affects tick abundance was the No. 1 outstanding uncertainty about Lyme listed in a 12-person consensus published in the June 5 Philosophical Transactions of the Royal Society B. Fighting Lyme disease appeals to Esvelt, who, like his pediatrician wife, grew up in the low-tick landscapes of the West Coast where Lyme is rare. In Massachusetts now, he says, “to both of us, it’s just horrific that a) there are that many ticks out there, and b) that they give you horrific diseases.” He especially regrets that neither of his two kids, nor anyone else’s, can tromp around outdoors, like he used to, carefree.
Esvelt calls the work of his lab, which plans to engineer a Lyme-resistant mouse, “sculpting evolution.” He and colleagues aim to tackle big biological problems like Lyme spread by using the insights of evolutionary biology plus the powerful gene-editing tool known as CRISPR/Cas9 (SN: 9/3/16, p. 22). But Esvelt wants to use that power with a startling openness and extreme public oversight.
“Right now, people don’t trust scientists to ensure that technologies are well understood before throwing them out there,” he says. “We have to fix that somehow.”
Before he even started to create a Lyme-resistant mouse in the lab, he asked for public meetings on the two Massachusetts islands where he hopes to test mice: Martha’s Vineyard and Nantucket. He got the green light to begin from citizen steering committees on both islands. But they still have the power to shut down the tests at milestones in the project. If the citizens nix the idea, he will walk away.
Originally Esvelt planned to sculpt Lyme disease into insignificance by acting on the ticks directly, driving down their numbers or changing them to be less dangerous. “But I talked to a lot of tick biologists who said, ‘Look, it’s not gonna happen.’ ” The black-legged ticks take so long to reproduce that the plan would only succeed “if you’re willing to wait about 50 years,” he says.
It’s actually faster to work with a mammal, the white-footed mouse. For the first tests, on islands, he plans great caution. He won’t even use a gene drive, the powerful way of deploying CRISPR/Cas9 so it overrides chancy natural inheritance and passes the desired genes to all offspring (SN: 12/12/15, p. 16). Instead he’ll just release mice genetically tweaked to be bad transmitters of Lyme and let natural mouse powers spread the genes.
Those mice won’t even be transgenic: They won’t carry genes from any other species. He’ll vaccinate island-captured mice in the lab, with an anti-Lyme vaccine or one that should confer an active immune response to tick bites. Then he’ll identify genes that produce the most protective reaction and put a large selection of them into what should be a safer animal that’s still “100 percent mouse,” he says.
While he’s tailoring safer mice for the island, however, he’s imagining new gene drives for a larger, mainland campaign. The way forward may require making gene drives less powerful, so they sputter out after a certain number of generations — “daisy chains,” he calls them, with loosely linked elements that fall apart easily. Going for the gut Ticks themselves probably have weaknesses that people haven’t yet exploited. The study of microbes in human guts has revolutionized ideas about human health and physiology. So Yale University’s Sukanya Narasimhan and Erol Fikrig are looking deep into the microbiome of the tick gut. Narasimhan describes the gut as a many-branched thing, “like a glove.” Ticks do have consistent bacterial residents, which could perhaps be exploited, but interactions look complex.
Along with Lyme, black-legged ticks can deliver other unpleasantries, such as human granulocytic anaplasmosis. When Anaplasma pathogens first tumble into a tick gut, invasion isn’t easy because some resident microbes form a biofilm along the gut lining that may be hard to breach. The pathogen, however, makes the tick secrete what’s essentially antifreeze, Fikrig, Narasimhan and colleagues reported in the Jan. 31 Proceedings of the National Academy of Sciences. The secretions can prevent biofilms from forming and ease the way for pathogen infection.
The sex lives of ticks could offer opportunities for completely different kinds of defenses, says longtime tick specialist Daniel Sonenshine of Old Dominion, author of Biology of Ticks.
He imagines, for instance, protecting livestock or dogs with decoys, “little bits of plastic” treated with a chemical cocktail that includes 2,6-dichlorophenol. That’s the come-hither substance female lone star and some other ticks release when they grab a mammal for a blood-feed. Like drinking venues for our species, mammals provide ticks with hot spots for finding mates. “These little plastic devices mimic a female tick,” Sonenshine says. And believe it or not, plastic fooled males long enough for a pesticide on the decoy to kill the ticks. (Tick sex on humans is possible but not likely, Gaff says. Humans rarely carry enough ticks at once to generate much of a scene.)
Robot vs. tick Tick biology is also important in designing a robot army. The concept behind TickBot came out of a collision of two very different visions of pest-fighter robotics.
As Gaff tells the story, engineers at the Virginia Military Institute in Lexington, “were under this mistaken idea … that ticks live in trees and they fall on your head.” The engineers’ solution: Use lasers to shoot ticks out of trees.
When they called to enlist Sonenshine in the project, he had to break the bad news: no blasting into shrubbery; ticks are on the ground. His advice: Don’t build a robot to attack ticks at all. Get the ticks to attack the robot. Instead of a laser-shooting, macho terminator, the concept morphed into a panting raccoon-sized machine that kills with dragged strips of pesticide-carrying cloth. The engineers’ four-wheeled buggy chugs slowly along following a looped guideline set up along a trail or in an open area.
As the bot works its way along, its motion, in some cases the passing shadow, will trick a tick into jumping at the cloth as it would the fur of a mouse or the sock of a hiker. But the big pull is carbon dioxide. Pest ticks “act like very lazy teenagers who don’t move unless they’re prodded,” says James Squire, one of the Military Institute engineer designers. Adding CO2 gets ticks’ attention and strengthens the illusion of a breathing, warm-blooded something.
The engineers — Squire, David Livingston and Gerald Sullivan — “came up with this amazing system of tubing and carbon dioxide canisters” for releasing CO2 from the bot, Gaff says. However, a student last year put a bit of dry ice in a cup with holes to mimic mammal breath. It worked and made the bot far easier to lug around.
Gaff remembers when she was first pulled in to do the field testing. “I was a huge skeptic coming in,” she says. To test TickBot outdoors, she chose as the first site a path through a wooded park with what she calls “infinite ticks.” There weren’t many Lyme ticks there to test, but the creeping cart tricked so many of the abundant lone star ticks that — high praise from a tick scientist in summer — she sat on the ground and had lunch.
Within a day, however, more ticks moved onto the cleared path from the nearby woods, she and colleagues reported in 2015 in Ticks and Tick-borne Diseases. Still, the notion of robotic tick catchers may be catching on. Gregory Gray, who teaches at Duke University’s Global Health Institute, worked with students from the local robotics club to design their own creeping, cloth-dragging cart.
And the TickBot team is now planning a bigger and faster robot that might ease the uncomfortable business of monitoring for cattle ticks on grazing land. Usually, people “dress up in woolly pajamas” as Squire puts it, and move through brush with an eye out for rattlesnakes in the Texas summer heat. The ticks grab the suit and are later counted. There’s got to be a better way.
For the TickBot itself, summer 2017 brought testing on grass around a playground. Like a little Roomba vacuum cleaner (but with guide wires), it set out twice a week to carve a safe zone by whisking away ticks, Gaff says.
“I’m giving them their space, and I’m asking them to respect my space,” she says. It’s all part of the mind-set of surrendering to the notion that there will always be ticks. But someday maybe we won’t care as much.
Add a new ingredient to the sugar, spice and everything nice needed to make girls.
A protein called COUP-TFII is necessary to eliminate male reproductive tissue from female mouse embryos, researchers report in the Aug. 18 Science. For decades, females have been considered the “default” sex in mammals. The new research overturns that idea, showing that making female reproductive organs is an active process that involves dismantling a primitive male tissue called the Wolffian duct. In males, the Wolffian duct develops into the parts needed to ejaculate sperm, including the epididymis, vas deferens and seminal vesicles. In females, a similar embryonic tissue called the Müllerian duct develops into the fallopian tubes, uterus and vagina. Both duct tissues are present in early embryos.
A study by French endocrinologist Alfred Jost 70 years ago indicated that the testes make testosterone and an anti-Müllerian hormone to maintain the Wolffian duct and suppress female tissue development. If those hormones are missing, the Wolffian duct degrades and an embryo by default develops as female, Jost proposed.
That’s the story written in textbooks, says Amanda Swain, a developmental biologist at the Institute of Cancer Research in London. But the new study “demonstrates that females also have a pathway to make sure you don’t get the wrong ducts,” says Swain, who wrote a commentary in the same issue of Science.
Testing Jost’s hypothesis wasn’t what reproductive and developmental biologist Humphrey Yao and colleagues set out to do. Instead, the researchers wanted to learn how tissues on the outside of the early ducts communicate with the tubes’ lining, says Yao, of the National Institute of Environmental Health Sciences in Research Triangle Park, N.C.
The COUP-TFII protein is produced in that outer layer, and Yao suspected it was involved in talking with the lining. The researchers blocked the communication in early female mouse embryos’ reproductive tissue by removing the gene that produces COUP-TFII. To the team’s surprise, the Wolffian duct remained in the female mice along with the female Müllerian duct. That shouldn’t happen, according to the textbooks. “We were just scratching our heads,” Yao says.
Searching for an explanation, Yao and colleagues first tested whether removing COUP-TFII changed the ovaries to produce testosterone like testes do. Testosterone could feed the male tissue and allow it to persist, the researchers thought.
“No, the ovary is just like an ovary. There’s nothing wrong with it,” Yao says. “We were just shocked. This can’t be happening.” Further experiments demonstrated that no stray testosterone was responsible for the male tissue sticking around.
Instead, COUP-TFII appears to be the foreman of a biochemical wrecking crew that demolishes the Wolffian duct in females. Without the protein barking orders, the demolition crew is idle and the male duct isn’t torn down. Signals that trigger COUP-TFII production and activity aren’t yet understood.
“This study fills a void in our understanding of the mechanism of regression of the Wolffian duct,” reproductive biologists Patricia Donahoe and David Pepin of Harvard Medical School said in an e-mail. More research is needed to understand how the protein interacts with male hormones to regulate reproductive tract development, they say.
While the study used mice, COUP-TFII probably works the same way in other mammals, including humans, Donahoe says. Females rarely still carry Wolffian duct remnants, sometimes leading to tumors. The opposite sometimes happens, too, resulting in males with female reproductive organs. Those men may be infertile and have other problems, such as cysts. Researchers should look for defects in COUP-TFII in patients with reproductive problems, Donahoe says.
